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Original Research Article | OPEN ACCESS

Comparative Antioxidant, Antiproliferative and Apoptotic Effects of Ilex laurina and Ilex paraguariensis on Colon Cancer Cells

Juan M Pérez1, Maria E Maldonado2, Benjamín A Rojano3, Fernando Alzate4, Jairo Sáez1, Wilson Cardona1

1Grupo de Química de Plantas Colombianas, Instituto de Química; 2Grupo de Impacto de los Componentes en la Salud, Escuela de Nutrición y Dietética, Universidad de Antioquia, A.A. 1226 Medellín; 3Grupo Ciencia de los Alimentos, Facultad de Ciencias, Universidad Nacional de Colombia. A.A. 1027 Medellin; 4Grupo de Estudios Botánicos, Instituto de Biología, Facultad de Ciencias Exactas y Naturales, Universidad de Antioquia, A.A. 1226 Medellín, Colombia.

For correspondence:-  Wilson Cardona   Email: wilson.cardona1@udea.edu.co   Tel:+5742195653

Received: 30 August 2013        Accepted: 17 June 2014        Published: 18 August 2014

Citation: Pérez JM, Maldonado ME, Rojano BA, Alzate F, Sáez J, Cardona W. Comparative Antioxidant, Antiproliferative and Apoptotic Effects of Ilex laurina and Ilex paraguariensis on Colon Cancer Cells. Trop J Pharm Res 2014; 13(8):1279-1286 doi: 10.4314/tjpr.v13i8.12

© 2014 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To determine and compare the antioxidant, antiproliferative and apoptotic effects of leaf infusions of Ilex laurina and Ilex paraguariensis in colon cancer cells.
Methods: Antioxidant activity was determined by ORAC (Oxygen Radical Absorbance Capacity) and FRAP (Ferric Reducing Antioxidant Power). Cytotoxic and antiproliferative effects were analyzed using MTT ((3-(4, 5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide) and sulfhorodamine-B respectively. Cell death and apoptosis of human colon adenocarcinoma cells SW480 and their metastatic-derived SW620 cells, were analyzed by flow cytometry using propidium iodide and Annexin-V.
Results:  Although their flavonoid levels were similar, I. laurina infusion contained 2.2 and 4.4 times higher amounts of total phenolic and caffeoyl derivatives, respectively, than I. paraguariensis. FRAP and ORAC values for I. laurina infusion were 1.6 and 2.0 more active than I. paraguariensis. Both plant infusions inhibited viability and cell growth of SW480 and SW620 cells. These results may be associated to cell cycle-arrest and apoptosis because of the comparable increase of hypodiploid and annexin-V positive colon cancer cells.
Conclusion: These data highlight the antioxidant and promising anticancer activities of I. laurina and Ilex paraguariensis.

Keywords: Ilex laurina, Ilex paraguariensis, Antioxidant, Antiproliferative, Apoptosis, Colon cancer

Impact Factor
Thompson Reuters (ISI): 0.523 (2021)
H-5 index (Google Scholar): 39 (2021)

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